When panning for antibodies against epitopes that impose a strong selective pressure for non-specific binders, the resulting molecules may exhibit poor binding specificity and unsatisfactory pharmacokinetic (PK) profiles. Highly-charged epitopes are a key example, where charge-based interactions saturate the binding pool. A leading pharmaceutical company was developing a high-affinity antibody for the highly-charged interleukin-21 receptor (IL-21R), an important player in the immune system’s cytokine response1. By leveraging Twist Bioscience’s Combinatorial Variant Library (CVL) Technology in combination with structure-guided library design and model-based selection, the company was able to enrich functional antibody variants with desirable characteristics and identify a stable lead with high-affinity for IL-21R.

Recommended handling and storage instructions for Twist Hight Throughput IgG

Twist High Throughput Antibody Production is a gene-to-protein workflow that can produce tens to thousands of diverse antibodies in small-scale quantities for discovery and screening purposes. This document will guide you through the process of preparing your sequences and placing an order within Twist’s ordering platform. The workflow starts with choosing VHH-Fc or conventional IgG antibody expression and submission of the desired antibody sequences into Twist’s intuitive ecommerce platform. Bioinformatic screening ensures that potential sequence liabilities that may affect antibody expression are mitigated. Once the order is received, Twist will synthesize and clone antibody genes into a vector of your choosing (either a custom vector or one of Twist’s IgG scaffold vectors). Twist antibody engineers will then express, affinity purify, and quality control the requested antibodies.