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Twist Alliance Canine Exome - 40.5 MB
Language English
Content
For Research Use Only. Not for use in diagnostic procedures
Understanding the genetic variation between dog breeds is critical to understanding genetic regulation of traits as well as the basis and progression of disease in dogs. Consequently, comprehensive gene panels such as exomes are pivotal in improving veterinary diagnostics and the associated clinical medicine. These types of NGS panels will also accelerate research into developing a deeper understanding of canine cancers and potential therapeutics.
The Twist Alliance Canine Exome developed in collaboration with the Broad Institute’s Karlsson lab provides just the right tool for canine genomic research. In addition, canine genomic research has also been found to have benefits for human medical research due to genetic similarities found between human and canine tumors such as CNVs, differential gene expressions, and structural chromosome abnormalities1.
The Twist Alliance Canine exome panel was designed with the following objectives in mind
- Covers coding exons of canine genes
- Enable comparative genomic studies between canine and human genomes
- Includes regions of known importance in human cancers
- Allows for cost-effective deep sequencing
The panel contains more than 17k Genes/targets based on CanFam3.1, with additional coverage of genes/targets that are implicated in canine cancer.
| GENES | ASSOCIATED COSMIC MUTATION | GENES | ASSOCIATED COSMIC MUTATION | GENES | ASSOCIATED COSMIC MUTATION |
| ABL1 | 19 | HRAS | 26 | TET2 | 16 |
| AKT1 | 6 | MPL | 0 | FAS | 3 |
| ERBB2 | 19 | SMAD4 | 31 | STAT5B | 4 |
| GNAS | 12 | ATM | 24 | DNMT3A | 6 |
| MET | 18 | FBXW7 | 25 | STAT3 | 6 |
| RB1 | 18 | IDH1 | 15 | CREBBP | 9 |
| CCR4 | 3 | NOTCH1 | 20 | RHOA | 1 |
| MYC | 5 | SMARCB1 | 11 | CHEK2 | 5 |
| EGFR | 123 | BRAF | 77 | PRDM1 | 6 |
| GNAQ | 6 | FGFR1 | 2 | CDKN2A | 108 |
| KRAS | 63 | IDH2 | 12 | FGFR3 | 17 |
| PTPN11 | 28 | NPM1 | 28 | JAK3 | 6 |
| CD28 | 3 | SMO | 5 | PDGFRA | 26 |
| PLCG1 | 3 | CDH1 | 7 | STK11 | 22 |
| ALK | 8 | FGFR2 | 8 | CTNNB1 | 73 |
| ERBB4 | 13 | JAK2 | 5 | GNA11 | 5 |
| HNF1A | 10 | NRAS | 35 | KIT | 139 |
| MLH1 | 1 | SRC | 1 | PTEN | 146 |
| RET | 17 | JAK1 | 12 | VHL | 124 |
| APC | 164 | IL7R | 4 | CSF1R | 8 |
| EZH2 | 11 | IL2RG | 3 | FLT3 | 30 |
| TP53 | 1087 | TNFAIP3 | 9 | KDR | 11 |
| FYN | 3 | PIK3CA | 97 |
The above table shows 50 such targets along with COSMIC mutations associated with each of these genes (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5978258/) - these genes were tiled 2x for higher coverage. FOXA1 and TERT promoter were tiled at 4x. All other regions were tiled at 1x, as per our standard practices.
With curated content from the leading canine cancer researchers in the world combined with Twist's robust panel performance, the Twist Alliance Canine Exome provides the most optimal tool for researchers studying canine diseases as well as rare human diseases.
1 Overgaard,N.H.,Fan,T.M.,Schachtschneider,K.M.,Principe,D.R.,Schook,L.B.,& Jungersen, G. (2018). Of Mice, Dogs, Pigs, and Men: Choosing the Appropriate Model for Immuno-Oncology Research. ILAR journal, 59(3), 247–262.
Note: The Twist Alliance Canine Exome - 40.5 MB is not ISO-13485 certified.
Ordering Information
105305 Twist Alliance Canine Exome - 40.5 Mb, 2 Reactions, Kit
105306 Twist Alliance Canine Exome - 40.5 Mb, 12 Reactions, Kit
105307 Twist Alliance Canine Exome - 40.5 Mb, 96 Reactions, Kit
