Nanobodies: Small but Mighty
The unique properties of VHHs (single-domain antibodies, scAbs, or nanobodies) overcome these challenges, making them a promising antibody format for therapeutics, reagents, or diagnostics. Regardless of your application, we offer comprehensive capabilities leveraging in vivo, in vitro, and in silico workflows to provide you with VHH antibodies with desired specificities and functional properties such as affinities in single-digit and subnanomolar ranges.
Flexible, Customizable Workflows for VHHs
In Vivo Workflows: Superior Targeting of Cell Surface
Proteins with Rapid Turnaround Times
Screen with speed
Twist has developed an optimized in vivo discovery workflow based on immunized camelids tailored for cell surface targets. We leverage an industry-leading, Beacon-based, single B cell screening platform, enabling the rapid identification of binders with required activities and/or cross-reactivity in 1 day.
Never miss a hit
We screen IgG2/3-secreting alpaca B cells for specificity and function using a high-resolution screening cascade that includes up to four on-cell assays before sequence recovery. Integrated cell line development services enhance our streamlined workflow, ensuring efficient and effective assays and outcomes.
Sans redevances
Twist also offers royalty-free, flexible business models to accommodate different partners' needs, including fee-for-service and equity-for-service structures.
Nanobodies: Small but Mighty
The unique properties of VHHs (single-domain antibodies, scAbs, or nanobodies) overcome these challenges, making them a promising antibody format for therapeutics, reagents, or diagnostics. Regardless of your application, we offer comprehensive capabilities leveraging in vivo, in vitro, and in silico workflows to provide you with VHH antibodies with desired specificities and functional properties such as affinities in single-digit and subnanomolar ranges.
Flexible, Customizable Workflows for VHHs
In Vivo Workflows: Superior Targeting of Cell Surface
Proteins with Rapid Turnaround Times
Screen with speed
Twist has developed an optimized in vivo discovery workflow based on immunized camelids tailored for cell surface targets. We leverage an industry-leading, Beacon-based, single B cell screening platform, enabling the rapid identification of binders with required activities and/or cross-reactivity in 1 day.
Never miss a hit
We screen IgG2/3-secreting alpaca B cells for specificity and function using a high-resolution screening cascade that includes up to four on-cell assays before sequence recovery. Integrated cell line development services enhance our streamlined workflow, ensuring efficient and effective assays and outcomes.
Sans redevances
Twist also offers royalty-free, flexible business models to accommodate different partners' needs, including fee-for-service and equity-for-service structures.
In Vitro Workflows: Ready-to-Use or Custom Discovery Libraries
Twist Biosciences’ precision DNA synthesis enables researchers to explore a vast, pre-defined sequence that focuses explicitly on the VHH sequences with the best chance of success.
Novel approach
We use multiple novel VHH sequence incorporation that combine synthetic and natural approaches to maximize diversity, creating high-quality, off-the-shelf VHH libraries for use against any target.
Unique and proprietary
These proprietary, naïve single-domain libraries are ideal for initiating antibody discovery and development and have been validated against immuno-oncology, CAR-T, GPCR, ion channel, and transporter targets.
Customization when you need it
We can also design and generate highly-diverse custom VHH libraries tailored to your program's needs in as little as 3 to 4 weeks.
Immune Library
Our scientists work closely with you to develop a custom VHH antibody discovery strategy that combines our in vitro and in vivo capabilities. We immunize camelids with the target antigen and isolate peripheral blood lymphocytes. The isolated lymphocytes are then used to construct a unique library of VHH antibody sequences. Our skilled scientists provide a custom library with high diversity and optimal biophysical properties, ensuring specificity and affinity.
VHH Optimization, Expression, and Characterization Under One Roof
In Vitro Workflows: Ready-to-Use or Custom Discovery Libraries
Twist Biosciences’ precision DNA synthesis enables researchers to explore a vast, pre-defined sequence that focuses explicitly on the VHH sequences with the best chance of success.
Novel approach
We use multiple novel VHH sequence incorporation that combine synthetic and natural approaches to maximize diversity, creating high-quality, off-the-shelf VHH libraries for use against any target.
Unique and proprietary
These proprietary, naïve single-domain libraries are ideal for initiating antibody discovery and development and have been validated against immuno-oncology, CAR-T, GPCR, ion channel, and transporter targets.
Customization when you need it
We can also design and generate highly-diverse custom VHH libraries tailored to your program's needs in as little as 3 to 4 weeks.
Immune Library
Our scientists work closely with you to develop a custom VHH antibody discovery strategy that combines our in vitro and in vivo capabilities. We immunize camelids with the target antigen and isolate peripheral blood lymphocytes. The isolated lymphocytes are then used to construct a unique library of VHH antibody sequences. Our skilled scientists provide a custom library with high diversity and optimal biophysical properties, ensuring specificity and affinity.
VHH Optimization, Expression, and Characterization Under One Roof
Optimisation et ingénierie des anticorps
- Rapid, precise creation of custom antibody libraries tailored to enhancing your therapeutic antibody candidates
- Affinity maturation: Rapid, high-diversity in silico screening to deliver variants with improved affinity
- Humanization: Service to engineer humanized antibodies, maintaining desired affinity, safety, and efficacy profiles
Express Antibody Production and Sequencing
- Small- to medium-scale HT VHH-Fc production: CHO (13-18 days), HEK (10-15 days)
- Bispecific matrixing: Reformatting scFv, Fab, bi- and multi-specifics to design and generate complex molecular formats
- NGS sequencing: AI/ML support
Caractérisation des anticorps
- Binding confirmation: ELISA, flow cytometry, Octet
- Affinity ranking and KD determination: SPR, Octet, flow cytometry
- Epitope binning: SPR, Octet
- Functional and bioanalytical: Internalization, cytotoxicity, ligand blocking, endotoxin testing, recruitment or inhibition, cross-reactivity
- Developability assessment: In silico analysis, CIC, AC-SINS, HIC, BVP-ELISA, aSEC, stability
Ressources techniques
Ressources techniques
Optimisation et ingénierie des anticorps
- Rapid, precise creation of custom antibody libraries tailored to enhancing your therapeutic antibody candidates
- Affinity maturation: Rapid, high-diversity in silico screening to deliver variants with improved affinity
- Humanization: Service to engineer humanized antibodies, maintaining desired affinity, safety, and efficacy profiles
Express Antibody Production and Sequencing
- Small- to medium-scale HT VHH-Fc production: CHO (13-18 days), HEK (10-15 days)
- Bispecific matrixing: Reformatting scFv, Fab, bi- and multi-specifics to design and generate complex molecular formats
- NGS sequencing: AI/ML support
Caractérisation des anticorps
- Binding confirmation: ELISA, flow cytometry, Octet
- Affinity ranking and KD determination: SPR, Octet, flow cytometry
- Epitope binning: SPR, Octet
- Functional and bioanalytical: Internalization, cytotoxicity, ligand blocking, endotoxin testing, recruitment or inhibition, cross-reactivity
- Developability assessment: In silico analysis, CIC, AC-SINS, HIC, BVP-ELISA, aSEC, stability
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