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protocol-b

Protocol

NGS Library Preparation For Target Enrichment With Covaris FFPE DNA Extraction

Application of Covaris Adaptive Focused Acoustics AFA technology during FFPE extraction provides consistently high yields of gDNA from challenging FFPE samples. The protocol provided here for FFPE extraction, shearing, and library preparation has been optimized to leverage the benefits of the Covaris system and generate gDNA libraries that provide excellent results upon target enrichment with Twist’s Target Enrichment System and Twist Mechanical Fragmentation Library Prep Kit.